EC HPLC column (analytical), NUCLEODUR 300-5 C4 ec, 5 µm

Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.

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Technical Data

 760193.20  760193.30  760193.40  760193.46  760194.20  760194.30  760194.40  760194.46
Column length 100 mm 125 mm
Column inner diameter 2 mm 3 mm 4 mm 4.6 mm 2 mm 3 mm 4 mm 4.6 mm
Column volume 0.3 mL 0.7 mL 1.25 mL 1.65 mL 0.37 mL 0.87 mL 1.56 mL 2.06 mL

 

 760195.20  760195.30  760195.40  760195.46  760196.20  760196.30  760196.40  760196.46
Column length 150 mm 250 mm
Column inner diameter 2 mm 3 mm 4 mm 4.6 mm 2 mm 3 mm 4 mm 4.6 mm
Column volume 0.45 mL 1.05 mL 1.9 mL 2.5 mL 0.8 mL 1.75 mL 3.15 mL 4.15 mL

 

Similar Details  760193.20, 760193.30, 760193.40, 760193.46, 760194.20, 760194.30, 760194.40, 760194.46, 760195.20, 760195.30, 760195.40, 760195.46, 760196.20, 760196.30, 760196.40, 760196.46
Method HPLC, analytical
Brand NUCLEODUR
Base material NUCLEODUR – high purity silica
Phase NUCLEODUR C4 ec 300-5
USP listing L26
Surface chemistry Medium density monomeric butyl (C4) wide-pore modification
Mode Reversed phase (RP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) Biological macromolecules, HPLC, Peptides, Proteins
Endcapped Yes
Carbon content 2.50%
pH stability 1.0–9.0
Pore size 300 Å
Specific surface according to BET 100 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 450 bar / 6525 psi
Hazardous material No

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